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pv-cre:ai-32 mice  (Jackson Laboratory)

 
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    Structured Review

    Jackson Laboratory pv-cre:ai-32 mice
    A: We used PV-Cre x Ai-32 mice (n = 18, 9 female) with surgically affixed headbars on their skulls, and craniotomies over the striatum. These mice expressed <t>ChR2</t> (EYFP) in PV+ neurons across the brain including the striatum, as visualized in a representative brain slice under fluorescence microscopy (inset). During each recording session, we lowered an optrode into a craniotomy targeting either the dorsal striatum (dStr) or the ventral striatum (vStr) for simultaneous electrophysiological recording and optical stimulation with blue light. B: Recording locations were determined based on a combination of craniotomy coordinates, recording depths, and histology, and classified as either from dorsal striatum (dStr, green square,depth: 2.5 ‒ 3.5 mm), or ventral striatum (vStr, red square, 3.5 ‒ 4.5 mm deep).
    Pv Cre:Ai 32 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pv-cre:ai-32 mice/product/Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    pv-cre:ai-32 mice - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Optogenetic mapping of rhythmic phase-dependent excitability in the mouse striatum"

    Article Title: Optogenetic mapping of rhythmic phase-dependent excitability in the mouse striatum

    Journal: bioRxiv

    doi: 10.1101/2024.02.01.578473

    A: We used PV-Cre x Ai-32 mice (n = 18, 9 female) with surgically affixed headbars on their skulls, and craniotomies over the striatum. These mice expressed ChR2 (EYFP) in PV+ neurons across the brain including the striatum, as visualized in a representative brain slice under fluorescence microscopy (inset). During each recording session, we lowered an optrode into a craniotomy targeting either the dorsal striatum (dStr) or the ventral striatum (vStr) for simultaneous electrophysiological recording and optical stimulation with blue light. B: Recording locations were determined based on a combination of craniotomy coordinates, recording depths, and histology, and classified as either from dorsal striatum (dStr, green square,depth: 2.5 ‒ 3.5 mm), or ventral striatum (vStr, red square, 3.5 ‒ 4.5 mm deep).
    Figure Legend Snippet: A: We used PV-Cre x Ai-32 mice (n = 18, 9 female) with surgically affixed headbars on their skulls, and craniotomies over the striatum. These mice expressed ChR2 (EYFP) in PV+ neurons across the brain including the striatum, as visualized in a representative brain slice under fluorescence microscopy (inset). During each recording session, we lowered an optrode into a craniotomy targeting either the dorsal striatum (dStr) or the ventral striatum (vStr) for simultaneous electrophysiological recording and optical stimulation with blue light. B: Recording locations were determined based on a combination of craniotomy coordinates, recording depths, and histology, and classified as either from dorsal striatum (dStr, green square,depth: 2.5 ‒ 3.5 mm), or ventral striatum (vStr, red square, 3.5 ‒ 4.5 mm deep).

    Techniques Used: Slice Preparation, Fluorescence, Microscopy



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